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. 2003 Sep;23(17):6129–6138. doi: 10.1128/MCB.23.17.6129-6138.2003

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FIG. 4. — Non-heat-shock genes are transcribed in tws^P mutants after heat shock. Salivary glands isolated from wild-type (A) and tws^P mutant (B) larvae were heat shocked for 20 min at 37°C in Grace's medium containing bromouridine (BrUTP). Polytene chromosomes were then immunostained for the presence of BrUTP (red) and Ser10-phosphorylated histone H3 (phospho-H3, green). Chromosome spreads were also counterstained with DAPI (white) to view the DNA. DAPI counterstain information is represented as blue in merged images. Visible heat shock loci are indicated in all panels. Strong regions of labeling that do not contain heat shock genes are indicated with an asterisk. Merged images indicate that numerous non-heat-shock gene loci containing the Ser10-phosphorylated histone H3 isoform in tws^P mutants are also actively transcribed after heat shock.