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. 2005 Jun;140(3):427–435. doi: 10.1111/j.1365-2249.2005.02785.x

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© 2005 British Society for Immunology

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Fig. 6 — Effects of untreated (▪) and anti-interleukin (IL)-1β (□) and anti-tumour necrosis factor (TNF)-α () antibody-treated CM from bacterial extracts-stimulated macrophages in the leptin released by adipocytes from SJL (a) and C57BL/6 (b) mice. Adipocytes were incubated with various bacterial extracts-treated macrophage supernatants, treated previously with monoclonal IL-1β and TNF-α, for 24 h. Leptin levels from adipocyte supernatants were evaluated by an enzyme-linked immunosorbent assay (ELISA) test. Experiments were conducted in triplicate. These results are representative of two similar experiments. *P ≤ 0·05; **P ≤ 0·01; ***P ≤ 0·001.

Inline graphic — Effects of untreated (▪) and anti-interleukin (IL)-1β (□) and anti-tumour necrosis factor (TNF)-α () antibody-treated CM from bacterial extracts-stimulated macrophages in the leptin released by adipocytes from SJL (a) and C57BL/6 (b) mice. Adipocytes were incubated with various bacterial extracts-treated macrophage supernatants, treated previously with monoclonal IL-1β and TNF-α, for 24 h. Leptin levels from adipocyte supernatants were evaluated by an enzyme-linked immunosorbent assay (ELISA) test. Experiments were conducted in triplicate. These results are representative of two similar experiments. *P ≤ 0·05; **P ≤ 0·01; ***P ≤ 0·001.