FIG. 3.

The impaired MPA induction of IMD2 in a ppr2 strain does not involve the GRE. (A) Wild-type cells were transformed with plasmids carrying IMD2-lacZ fusions containing (P892) or not containing (P1200) the coding sequences of IMD2. Expression from these reporter plasmids was measured for wild-type cells grown to exponential phase and then treated for 12 h with hydroxurea, an inhibitor of ribonucleotide reductase that affects cellular proliferation. Gua, guanine; βGal, β-galactosidase. (B) Strains BY4742 (wild type [wt]) and Y14411 (PPR2 disruption [ppr2]) were transformed with the indicated plasmids (Fig. 2) and grown from exponential precultures for 6 h in the presence or absence of MPA. Cells were harvested and assayed for βGal activity.