Fig. 2.
Inhibition of Salmonella serovar Enteritidis-induced interleukin (IL-8) secretion in crypt-like (a) and villus-like (b) Caco-2 cells by heat shock treatment. Control (1 h 37°C/6 h 37°C) and heat-shocked cells (1 h 42°C/6 h 37°C) were exposed to graded numbers of bacteria for 1 h and allowed to recover from bacterial exposure for 24 h. Salmonella-induced interleukin (IL)-8 secretion is expressed as pg IL-8/106 cells. Inhibition of Salmonella-induced IL-8 secretion was determined using two cell passages and triplicate cultures per passage. Significant differences (*P < 0·05) between the IL-8 levels of heat-shocked and control Caco-2 cells are indicated.