Fig. 3.

Induction of heat shock protein (Hsp) 70 synthesis in enterocyte-like Caco-2 cells during incubation with butyrate. Cells were incubated for 48 h in a range of physiological butyrate concentrations. HS indicates the level of Hsp70 in cells heat-shocked at 42°C in the absence of butyrate. Subsequently, the cells were processed for Western blotting and immunostaining as described. The blot is a representative of two cell passages and triplicate cultures per passage.