Fig. 1.
Changes in cytokine production by PBMCs. Cells (106/ml) were obtained from patients with UC before and after their first LCAP and cultured with LPS (10 ng/ml) or medium alone (a) or with PHA (1/100 dilution) (b) for 24 h. Supernatants were collected and cytokine concentrations were measured using commercial ELISA kits. Data are expressed as the mean of duplicate samples. *P < 0·05. PBMC, peripheral blood mononuclear cell; UC, ulcerative colitis; LCAP, leukocytapheresis; LPS, lipopolysaccharide; PHA, phytohemagglutinin; ELISA, enzyme-linked immunosorbent assay.