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. 2005 Jul;141(1):130–140. doi: 10.1111/j.1365-2249.2005.02825.x

Fig. 3.

Fig. 3

Effects of LCAP on binding of the NF-κB subunit to an NF-κB-binding consensus sequence. PBMCs were obtained from normal subjects (n = 6) or UC patients before and after their first LCAP (n = 7) and cultured with LPS at 10 ng/ml for 24 h. Lysates of PBMCs (5 µg) were tested for binding of activated p65 NF-κB subunit to an NF-κB consensus sequence using the Trans-Am NF-κB ELISA kit. Results are expressed as absorbance values. *P < 0·05. OD, optical density; NF, nuclear factor; PBMC, peripheral blood mononuclear cell; ELISA, enzyme-linked immunosorbent assay; LPS, lipopolysaccharide; LCAP, leukocytapheresis.