Fig. 5.

(a)Macrophage inflammatory protein-1β (MIP-1β) protein determination in bronchoalveolar lavage (BAL) fluid of lipopolysaccharide (LPS)-normoxia and LPS-hypoxia animals. LPS, 150 µg, was instilled intratracheally and animals were exposed to normoxia or hypoxia for 2, 4, 6 and 8 h. Lungs were lavaged, and proteins were electrophoresed on a sodium dodecyl sulphate-polyacrylamide gel and transblotted to a nitrocellulose membrane. The blot represents one of five experiments. Densitometry was performed. Value for control animals was defined as 1 and all other values were adapted. Values are mean ± s.e.m. from five animals. *P < 0·05 between LPS-normoxia and LPS-hypoxia animals. (b) MIP-1β protein concentration in BAL fluid of alveolar macrophage-competent and -depleted animals, exposed to LPS-normoxia and LPS-hypoxia. Animals were pretreated with control liposomes (co lip) or clodronate-liposomes (clodr); 72 h later 150 µg LPS (L) was instilled intratracheally and animals were exposed to either normoxia (N) or hypoxia (H) for 6 h. BAL fluid proteins were electrophoresed on a SDS-polyacrylamide gel and transblotted to a nitrocellulose membrane. The blot represents one of five experiments.