Fig. 7.

Enzyme-linked immunosorbent assay (ELISA) for albumin content in bronchoalveolar lavage (BAL) fluid. Lipopolysaccharide (LPS), 150 µg, was instilled intratracheally and animals were exposed to normoxia or hypoxia for 2, 4, 6 and 8 h. Lungs were lavaged and albumin was analysed in BAL fluid. Values are means ± s.e.m. from five animals. *P < 0·05 between LPS-normoxia and LPS-hypoxia animals. (b) ELISA for albumin content in BAL fluid of alveolar macrophage-competent and -depleted animals, exposed to LPS-normoxia and LPS-hypoxia. Animals were pretreated with control liposomes (co lip) or clodronate-liposomes (clodr); 72 h later 150 µg LPS (L) was instilled intratracheally and animals were exposed to either normoxia (N) or hypoxia (H) for 6 h. The blot represents one of five experiments.