Fig. 4.

IL-15 enhances adhesion of neutrophils onto A549 cells. (a) neutrophils were stimulated with buffer (Ctrl), LPS, or IL-15 for 2 h, labelled with calcein AM, incubated on confluent A549 cells for 30 min and adhesion was measured as described in Methods. Results are means ± SEM (n = 4). (b) The adhesion assay was performed as in A, except that A549 cells were stimulated with buffer (Ctrl), TNF-α or IL-15 for 24 h. Results are means ± SEM (n = 3). Pictures on the right part of the figure illustrated typical data that were plotted for the graph. *P < 0·05 versus Ctrl by anova.