Table 2.
Oligonucleotidea | Position in message | |
---|---|---|
hGRα | ||
Forward primer12 | CCTAAGGACGGTCTGAAGAGC | 2158–2178 |
Reverse primer12 | GCCAAGTCTTGGCCCTCTAT | 2616–2635 |
Donor probe | TTGTCAGTTGATAAAACCGCTGCCAGTTCT-flu | 2290–2261 |
Acceptor probe | LC Red640-GCTGGAGTTTCCTTCCCTCTTGACAATG | 2259–2230 |
hGRβ | ||
Forward primer19 | ACACAGGCTTCAGGTATCTT | 2091–2110 |
Reverse primer12 | CCACGTATCCTAAAAGGGCAC | 2503–2523 |
Donor probe | TGTGCACTTCGTTGTCAATAATAAGTCAACT-flu | 2421–2451 |
Acceptor probe | LC Red640-ATGCTCATCGACAACTATAGGAGGCTTT | 2453–2480 |
Oligonucleotide sequences are 5′−3′.