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Migration of IgA+ and IgG+ memory B cells. Circulating CD19+ B cells were isolated from healthy volunteers and the migration of IgA+ (▪) and IgG+ (▴) cells towards increasing concentrations of (a) CXCL11 (b) CCL17, and (c) CCL25 was analysed in the transwell system, using flow cytometry to enumerate and characterize migrating cells. Assays were carried out in duplicates and the figure shows one representative experiment out of four.
