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Crb2 colocalizes with Rad22 to sites of DSBs. (A) YFP-Crb2 foci (red) and Rad22-CFP (green) foci induced by HO overlapped with each other. (B) YFP-Crb2 and Rad22-CFP partially colocalize to gamma radiation-induced foci. Rad22-CFP foci became brighter after 3 h of recovery; therefore, the diffuse nucleoplasmic signal is no longer visible after normalization of the image to the intensity of foci. Scale bar, 5 μm.
