Fig. 2.

Sam68 is tyrosine phosphorylated in peripheral blood mononuclear cells (PBMC) from HIV infected patients, mediating the formation of signalling complexes with STAT-3. Cells (PBMC) from HIV infected and control subjects were homogenized in lysis buffer and soluble supernatant immunoprecipitated with anti-Sam68 or anti-STAT-3 antibodies. Immunoprecipitation samples were then analysed by immunoblot with antiphosphotyrosine (a), anti-Sam68 (b) and anti-STAT-3 antibodies (c). Representative results are shown. Panels show the means ± s.e.m. of the quantitative data. *P < 0·001 versus control.