Fig. 4.

Sam68 expression is decreased in peripheral blood mononuclear cells (PBMC) from HIV infected patients. (a) Total RNA was isolated from control and patient PBMC. RNA was retrotranscribed and amplified by reverse transcription-polymerase chain reaction (RT-PCR) using primers that amplify KH region. Twenty cycles of PCR allowed the evidence for differences in the RNA levels. β-actin was amplified as a control of total RNA amount. The lower panel shows the means ± s.e.m. of the quantitative data. *P < 0·01 versus control. (b) Cells were homogenized in lysis buffer and soluble supernatants were normalized by protein concentration and analysed by Western blot using anti-Sam68 antibodies. Anti-β-actin immunoblot was employed to check the general amount of protein in the blots. The lower panel shows the means ± s.e.m. of the quantitative data. *P < 0·01 versus control.