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Indirect immunofluorescence of permeabilized human podocytes ‘in culture’ incubated with (a) serum of patients with circulating anti-actin and anti-ATP synthase β chain antibodies and (b) normal serum. After several PBS washings slides were incubated with FITC conjugated anti-human IgM antibodies. In the former case, diffuse cytoplasmatic staining of human IgM was observed.
