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IFNγ secretion in response to M. bovis specific antigens ESAT-6 and CFP-10. Calves were inoculated with M. avium (a–e) or sham inoculated (f–j). Twelve weeks later all calves were challenged with M. bovis. Responses were assessed bi-weekly post challenge (week 0). Whole blood was stimulated with ESAT-6 (□), CFP-10 (▴) or medium control (▪) and IFNγ secretion in supernatants was assessed by ELISA. Individual animal data is shown.
