Fig. 7.

Cytokine production in lungs of AdmIFN-γ-treated mice. Mice were treated with AdmIFN-γ, AdEGFP, saline 24 h before administration of Af i.n. On day 2 after Af treatment, mice were sacrificed for lung homogenate and lung cells. Lung cells were simply cultured in RPMI for 48 h. (a) IFN-γ (▪) and TNF-α (□) levels in lung homogenates and (b) IFN-γ (▪) and IL-12 (□) levels in culture supernatants of lung cells were measured by ELISA. The experiment was performed three times (n = 5 animals per time point). *P < 0·01 compared with the same cytokine levels in mice treated with saline and AdEGFP, respectively.