Table 2. Effect of vascular endothelial peptide (VIP) on cAMP accumulation in non-obese diabetic (NOD) salivary glands.

	cAMP (fmol/mg)
	Submandibular glands		Parotid glands
NOD mice age	Basal	VIP	Basal	VIP
10 weeks	171 ± 33	274 ± 19*	211 ± 3	442 ± 22*
14 weeks	238 ± 32	228 ± 21	202 ± 37	296 ± 21
16 weeks	222 ± 16	207 ± 34	240 ± 99	288 ± 55
BALB/c mice	233 ± 19	499 ± 69*	179 ± 19	528 ± 83*

Samples from NOD mice of the ages indicated and BALB/c mice (16 weeks of age) were prepared by incubating whole glands for 30 min in Krebs–Ringer bicarbonate (KRB), gassed with 5% CO₂ in O₂ and vascular endothelial peptide (VIP) (100 nM) was added during the last 15 min. Once homogenized in ethanol and dried, residues were dissolved in sodium-acetate buffer for subsequent cyclic nucleotides measurement by radioimmunoassay (RIA). Values are mean ± s.e. of at least six separate determinations.

^*P < 0·05 versus basal value of the corresponding gland at the same age.