TABLE 1.
Yeast strains used
| Strain | Genotype | Reference(s) or source |
|---|---|---|
| W303a/α | MATa/α leu2-3,112 his3-11,15 ade2-1 trp1-1 ura3-1 can1-100 | 19 |
| KSY601 | MATα leu2-3,112 his3-11,15 ade2-1 trp1-1 ura3-1 can1-100 tif6::HIS3/TIF6 | 26 |
| KSY603 | MATα leu2-3,112 his3-11,15 ade2-1 trp1-1 ura3-1 can1-100 tif6::HIS3 p[URA3 GAL10::Ub-HA-TIF6] | 2, 26 |
| KSY605 | MATα leu2-3,112 his3-11,15 ade2-1 trp1-1 ura3-1 can1-100 tif6::HIS3 p[TRP1 TIF6-Myc] | 2 |
| KSY606 | MATα leu2-3,112 his3-11,15 ade2-1 trp1-1 ura3-1 can1-100 tif6::HIS3 p[URA3 TIF6] | 2 |
| KSY607 | MATα leu2-3,112 his3-11,15 ade2-1 trp1-1 ura3-1 can1-100 tif6::HIS3 p[LEU2 TIF6-HA] | 2 |
| UBY608 | MATα leu2-3,112 his3-11,15 ade2-1 trp1-1 ura 3-1 can1-100 tif6::HIS3 p[TRP1 TIF6-Myc] p[LEU2 tif6-HA (S174A, S175A)] | This work |
| UBY609 | MATα leu2-3,112 his3-11,15 ade2-1 trp1-1 ura3-1 can1-100 tif6::HIS3 p[URA3 GAL10::Ub-HA-TIF6] p[LEU2 tif6-GFP(S174A, S175A)] | This work |
| UBY610 | MATα leu2-3,112 his3-11,15 ade2-1 trp1-1 ura3-1 can1-100 tif6::HIS3 p[TRP1 TIF6-Myc] p[LEU2 TIF6-HA] | This work |
| UBY611 | MATα leu2-3,112 his3-11,15 ade2-1 trp1-1 ura3-1 can1-100 tif6::HIS3 p[URA3 GAL10::Ub-HA-TIF6] p[LEU2 TIF6-GFP] | This work |
| UBY612 | MATα leu2-3,112 his3-11,15 ade2-1 trp1-1 ura3-1 can1-100 tif6::HIS3 p[URA3 TIF6] p[LEU2 GAL10::TIF6-GFP] | This work |
| UBY613 | MATα leu2-3,112 his3-11,15 ade2-1 trp1-1 ura3-1 can1-100 tif6::HIS3 p[URA3 TIF6] p[LEU2 GAL10::tif6-GFP(S174A, S175A)] | This work |