Table 2.
Secretion of IL-1β, TNF-α, IL-6, IFN-γ, IL-10 and PGE2 in human whole blood from six healthy individuals following stimulation with 107 cells/ml of P. gingivalis ATCC 33277 for 6 h.
| Individual | P. gingivalis ATCC 33277 (cells/ml) | PGE2 (pg/ml) | Amount of cytokine secreted (pg/ml) | ||||
|---|---|---|---|---|---|---|---|
| IL-1β | TNF-α | IL-6 | IFN-γ | IL-10 | |||
| 1 | 0 | ND | ND | ND | ND | 77 ± 22 | 37 ± 18 |
| 107 | 133 ± 11 | 1 478 ± 39 | 3 656 ± 233 | 6 005 ± 137 | 115 ± 26 | 110 ± 8 | |
| 2 | 0 | ND | ND | ND | ND | 143 ± 20 | 36 ± 15 |
| 107 | 67 ± 8 | 4 334 ± 234 | 5 616 ± 330 | 5 779 ± 293 | 304 ± 21 | 44 ± 11 | |
| 3 | 0 | ND | ND | ND | ND | 65 ± 3 | 43 ± 9 |
| 107 | 311 ± 23 | 7 688 ± 621 | 9 377 ± 276 | 10 556 ± 154 | 132 ± 23 | 307 ± 16 | |
| 4 | 0 | ND | ND | ND | ND | 60 ± 6 | 29 ± 1 |
| 107 | 96 ± 13 | 10 416 ± 316 | 7 189 ± 413 | 5 651 ± 179 | 195 ± 43 | 77 ± 8 | |
| 5 | 0 | ND | ND | ND | ND | 68 ± 13 | 26 ± 2 |
| 107 | 84 ± 7 | 10 325 ± 223 | 10 557 ± 201 | 7 435 ± 115 | 2239 ± 71 | 49 ± 7 | |
| 6 | 0 | ND | ND | ND | ND | 102 ± 5 | 41 ± 13 |
| 107 | 263 ± 16 | 5 180 ± 79 | 7 502 ± 162 | 12 721 ± 146 | 208 ± 35 | 287 ± 31 | |
| Mean | 0 | ND | ND | ND | ND | 85 ± 31 | 35 ± 6 |
| 107 | 159 ± 102* | 6 570 ± 3549* | 7 316 ± 2493* | 8 024 ± 2951* | 532 ± 838* | 145 ± 119 | |
The cytokine concentrations in the supernatants of the whole blood cultures were assessed by ELISA. PGE2 concentration was assessed by EIA. Control whole blood samples were incubated without bacteria. The data are the means ± SD of triplicate assays.
*
P < 0·05 compared to an uninfected control. ND: Not detected (below the detection level).