Table 3.
Secretion of IL-8, RANTES, MCP-1 and IP-10 in human whole blood from six healthy individuals following stimulation with 107 bacteria/ml of P. gingivalis ATCC 33277 for 6 h.
| Individual | P. gingivalis ATCC 33277 (cells/ml) | Amount of chemokine secreted (pg/ml) | |||
|---|---|---|---|---|---|
| IL-8 | RANTES | MCP-1 | IP-10 | ||
| 1 | 0 | ND | 565 ± 49 | 73 ± 5 | 443 ± 31 |
| 107 | 6 628 ± 408 | 2 848 ± 180 | 487 ± 15 | 1 395 ± 25 | |
| 2 | 0 | ND | 437 ± 57 | 97 ± 28 | 425 ± 50 |
| 107 | 2 845 ± 123 | 3 201 ± 101 | 1616 ± 206 | 15 257 ± 316 | |
| 3 | 0 | ND | 6 207 ± 290 | 60 ± 13 | 447 ± 38 |
| 107 | 20 981 ± 441 | 12 544 ± 237 | 3432 ± 211 | 1 025 ± 73 | |
| 4 | 0 | ND | 1 278 ± 79 | 86 ± 6 | 181 ± 24 |
| 107 | 9 864 ± 248 | 3 986 ± 194 | 3002 ± 439 | 9 150 ± 217 | |
| 5 | 0 | ND | 3 383 ± 201 | 44 ± 8 | 187 ± 27 |
| 107 | 7 891 ± 322 | 7 708 ± 192 | 2323 ± 135 | 12 431 ± 227 | |
| 6 | 0 | ND | 1 535 ± 43 | 103 ± 20 | 339 ± 31 |
| 107 | 21 842 ± 565 | 4 036 ± 141 | 2855 ± 872 | 950 ± 231 | |
| Mean | 0 | ND | 2 234 ± 2214 | 77 ± 22 | 337 ± 124 |
| 107 | 11 675 ± 7886* | 5 720 ± 3767* | 2285 ± 1080* | 6 701 ± 6410 | |
The chemokine concentrations in the supernatants of whole blood cultures were assessed by ELISA. Control whole blood samples were incubated without bacteria. The data are the means ± SD of triplicate assays.
*
P < 0·05 compared to an uninfected control. ND: Not detected (below the detection level).