Time course-changes in the extent of MAPKs phosphorylation during ethanol-induced gastric injury in rats. Gastric injury was induced by intragastric administration of 100% ethanol. Expression of phospho-ERK, ERK, phospho-p38, p38, phospho-JNK, and JNK in tissue homogenates was determined by Western blotting. A representative Western blot of three separate experiments is shown.