Table 2.
Percentages of leucocyte subsets before and after immunization.
| % Positive cells (range) | |||
|---|---|---|---|
| Parameter (marker) | Before immunization (day 1) (n = 15) | Immunized (day 74) (n = 15) | Pg |
| Lymphocytes (FSC/SSC)a | 14·5 (8·8–25·8) | 12·5 (5·1–25·2) | 0·155 |
| Monocytes (FSC/SSC)a | 7·5 (5·1–12·1) | 5·9 (3·1–9·2) | 0·019 |
| Granulocytes (FSC/SSC)a | 66·3 (50·7–77·1) | 70·7 (52·4–84·2) | 0·054 |
| B cells (CD19+)b | 6·6 (1·2–12·6) | 7·2 (3·4–11·0) | 0·716 |
| Memory B-cells (CD19+ CD27+)c | 26·6 (17·1–47·0) | 25·9 (14·8–45·0) | 0·763 |
| IgM+ memory B cells (IgM+CD27+)c | 10·1 (4·5–34·0) | 9·9 (3·0–29·1) | 0·931 |
| IgG+ memory B cells (IgG+CD27+)c | 3·1 (0·9–6·2) | 4·5 (2·5–9·6) | 0·074 |
| T cells (CD3+)b | 72·6 (64·2–82·0) | 72·0 (59·2–85·1) | 0·731 |
| CD4+T cells (CD3+CD4+)d | 61·8 (46·3–71·4) | 62·8 (49·8–72·7) | 0·545 |
| CD8+T cells (CD3+CD8+)d | 36·9 (28·6–53·7) | 36·5 (27·3–50·2) | 0·545 |
| Ratio CD4/CD8 | 1·7 (0·9–2·5) | 1·7 (1·0–2·7) | 0·828 |
| Naive CD4+T cells (CD8–CD45RA+)e | 31·6 (20·0–48·8) | 37·7 (24·5–65·4) | 0·008 |
| Naive CD8+T cells (CD8+CD45RA+)f | 45·9 (26·7–67·0) | 51·0 (28·8–78·1) | 0·095 |
a
Among all cells
b
among all lymphocytes (defined by FSC/SSC)
c
among all B cells (CD19+)
d
among all T cells (CD3+)
e
among all CD4 T cells (CD3+CD8–)
f
among all CD8 T cells (CD3+CD8+)
g
paired, two-tailed t-test between day 1 and day 74.