Fig. 3.

Expression of major histocompatibility complex (MHC) class II antigens and of CD83 on polymorphonuclear neutrophils (PMN): (a) with an antibody recognizing HLA-DP-DQ-DR, expression of MHC class II antigen was found on PMN from the lavage (middle panel), the drainage fluid (right panel) but not on cells derived from the peripheral blood (left panel) (the thick lines show the specific antibody; the thin lines the isotype controls; data for patient 2 are shown). Similarly, expression of CD83 was seen on PMN derived from the lavage or the drainage fluid, but not on peripheral blood PMN. (b) Data for all patients are summarized (as detailed in the legend for Fig. 2). (c) Reverse transcription-polymerase chain reaction (RT-PCR) was performed with PMN derived from the peripheral blood or from the lavage of the same patient (data for patient 4 are shown) with primers specific for either CD83, MHC class II or GAPDH as housekeeping gene.