Fig. 4.

Fast protein liquid chromatography profile of serum obtained from a patient with ulcerative colitis (a) and from a patient with Crohn's disease (b). Serum samples were fractionated by molecular sieving over a Superose 6 HR 10/30 column. Each fraction was assayed by enzyme-linked immunosorbent assays (ELISAs) for interleukin (IL)-6, soluble IL-6 receptor (sIL-6R) and soluble gp130 (sgp130), and results were plotted as immunoreactive concentrations versus fraction number. Elution positions of the molecular markers, thyroglobulin (669 kDa), IgG (150 kDa), BSA (67 kDa) and chymotrypsinogen A (25 kDa) indicated by arrows. The results are representative for five patients studied.