Fig. 1.

Cytofluorimetric dendritic cells (DC) subset definition by TruCOUNT assay. The R1 gate was used for lymphocytes and monocytes. Beads appear at the extreme right of the dot plot (a). The R2 gate was created to identify lineage-negative cells (b). To define myeloid DC (mDC) and plasmacytoid DC (pDC), events from R1 and R2 were analysed in a contour plot of CD11c versus human leucocyte antigen (HLA)-D-related (DR) (c) or CD123 versus HLA-DR (d). All CD11c^hi-HLA-DR⁺ and CD123^hi-HLA-DR⁺ were included in this gating strategy. TruCOUNT beads (R4) were obtained in an ungated dot plot of fluorescence ( FL)1 versus FL2 (e).