Fig. 2.

Monoclonal recombinantly cloned human IgM antibody (rHIgM12) promotes antigen uptake in CpG-matured murine dendritic cells. Bone marrow-derived dendritic cells (DC) were cultured in the presence of granulocyte–macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 for 5 days, at which time they were matured using 10 µg/ml CPG oligodeoxynucleotide. Twenty-four hours later, fluorescein isothiocyanate–ovalbumin and either control antibody (filled), sHIgM12 (solid line) or rHIgM12 (dotted line) was added to the culture. Following a 24-h incubation, the cells were stained with phycoerythrin-conjugated anti-mouse CD11c, fixed with 2% paraformaldehyde and analysed by flow cytometry on a FACSCalibur (BD Biosciences-PharMingen, San Diego, CA, USA). Data were analysed using CellQuest software. Histogram shows cells gated through CD11c.