FIG. 5.

Transgenic inducible cyclin D1 antisense regulates PPARγ abundance in vivo. Western blot of cyclin D1^−/− 3T3 (A) and cyclin D1^−/− MEFs (B) with antibodies as indicated. (Ca) An ecdysone enhancer-driven cyclin D1 antisense transgene (schematic shown), was used to generate transgenic mice (Tg) confirmed by genomic Southern blot (Cb). (Cc) Adenoviral vectors encoding receptors for the Bombyx LBD either with or without a second cistron for GFP were used to infect the cyclin D1 antisense transgenic mice (Cd). (Ce) Transgenic mice were treated with ponasterone to induce transgene expression, and Western blotting of hepatic extracts was performed at 5 days for the loading control (GDI), cyclin D1 abundance and GFP from the second cistron of the cyclin D1 antisense, and PPARγ.