Fig. 2.

Differences in gene expression between types I and II responders. (a) Significant differences in lipopolysaccharide (LPS)-mediated gene expression between type I and type II responders for genes implicated in inflammatory processes. Filled bars: type II responders, open bars: type I responders. The results are expressed as mean signal log ratio (SLR) (n = 5 for each responder type) ± s.d. (b) Validation of gene expression profiling was performed by reverse transcription–reverse polymerase chain reaction (RT-PCR) for interleukin (IL)-8, IL-6, monocyte chemoattractant protein (MCP)-1, E-selectin and intercellular adhesion molecule (ICAM)-1. Total RNA was isolated from unstimulated (−) or LPS (1 µg/ml, 24 h)-stimulated (+) cells. A representative experiment using two different types I and II responders is depicted. Similar findings were found in all types I and II responders.