Table 2.
Stimulation of interleukin (IL)-12 production by heat shock protein (HSP)70, CD40L or lipopolysaccharide (LPS) of mononuclear cells eluted from colonic biopsy specimens from patients with Crohn's disease or ulcerative colitis, and inhibition of HSP70-stimulated cells with p457–496 or pCD40/CD40L.
| HSP70-stimulated cells inhibited with | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Concentration of IL-12, mean ± s.e.m. pg/ml | P457-496 | PCD40/CD40L | |||||||
| Mucosal cells | n | HSP70 | CD40L | LPS | n | Pre- | Post- | Pre- | Post- |
| (a) Crohn's disease | |||||||||
| Uninflamed | 8 | 24·9 (10·2) | 39·9 (11·6) | 32·6 (11·9) | 8 | 20·3 (8·0) | 2·8 (1·8) | 20·3 (8·0) | 6·6 (3·8) |
| Inflamed | 10 | 28·1 (7·3) | 30·9 (12·4) | 58·0 (30·5) | 10 | 24·3 (8·0) | 6·6 (2·9) | 24·3 (7·2) | 11·5 (2·9) |
| P-value | 0·753 | 0·396 | 1·0 | UninflamedInflamed | 0·0610·022 | 0·0430·023 | |||
| (b) Ulcerative colitis | |||||||||
| Uninflamed | 6 | 6·1 (2·8) | 7·2 (5·1) | 16·2 (8·9) | 6 | 19·2 (12·1) | 4·5 (2·9) | 19·2 (12·1) | 5·0 (2·9) |
| Inflamed | 5 | 19·9 (12·7) | 3·4 (3·2) | 23·2 (14·4) | 5 | 17·3 (9·2) | 5·8 (4·2) | 17·3 (9·2) | 12·5 (7·2) |
| Uninflamed | 0·18 | 0·19 | |||||||
| Inflamed | 0·30 | 0·17 | |||||||