Fig. 2.
Decreased apoptosis in neutrophils of patients with the TLR4 (Asp299Gly) GG genotype. (a) apoptosis was measured by propidium iodide staining and FACS in 5 × 106 neutrophils incubated for 12 h in autologous serum with (▪) or without (□) LPS (10 µg/ml). Results represent the mean ± SD of at least three individuals. P = 0·034 while comparing the spontaneous apoptosis of OM patients with the TLR4 AA and GG genotypes. P = 0·038 for the LPS-induced apoptosis while comparing the TLR4 AA and AG versus GG genotypes. (b) apoptosis was determined by DNA laddering.