Table 1.

Oligonucleotide primer sequences, PCR conditions and restriction enzymes used for genotyping and sequencing of the three polymorphisms.

Gene	Polymorphism	Primers	PCR pr. length (bp)	Annealing temp (°C)	Restriction enzyme
TLR2	Arg753Gln	F: 5′-GAGTGGTGCAAGTATGAACTGGA-3′	260	62	Pst I
		R: 5′-TCCCAACTAGACAAAGACTGGTCT-3′
TLR4	Asp299Gly	F: 5′-GATTAGCATACTTAGACTACTACCTCCATG-3′	263	56	Nco I
		R: 5′-GATCAACTTCTGAAAAAGCATTCCCAC-3′
TLR4	Thr399Ile	F: 5′-TGGCAACATTTAGAATTAGTTAAC-3′	227	52	Msp I
		R: 5′-CTCAGATCTAAATACTTTAGGCCG-3′

The underlined bases in the primers differ from the original sequences and served to introduce a restriction site or to disrupt a natural restriction site within the primer sequence.