Table 1.
Antibody | Molecule | Source | Dilution | Incubation | Tissue | Antigen retrieval |
---|---|---|---|---|---|---|
CD25 | CD25 | Dako | 1 : 50 | 1 h | Frozen | Nil |
ICAM-1 | CD54 | Becton Dickinson | 1 : 90 | 1 h | Frozen | Nil |
HLA-DR | MHC II | Dako | 1 : 30 | Overnight, 4°C | Paraffin | Microwave |
Anti-tryptase | Mast cell tryptase | Dako | 1 : 50 | Overnight, 4°C | Paraffin | Pronase digestion |
Ki-67 | Ki-67 nuclear antigen | Dako | 1 : 80 | Overnight, 4°C | Paraffin | Microwave |