Fig. 5.

Immunostaining of renal tissue from normal rats and rats with anti-Thy1·1 GN. Kidney sections (4 µm) were immunostained for proliferating cell nuclear antigen (PCNA) and developed with 3,3-diaminobenzidine to produce a brown colour. (a) Normal, (b) anti-Thy1·1 glomerulonephritis treated with 10% dimethyl sulphoxide in saline at day 6 (Vehicle-treated GN), (c) anti-Thy1·1 glomerulonephritis treated with STI 571 at day 6. (STI-treated GN). (d) Quantification of Proliferating Cell Nuclear Antigen positive glomerular cells. Fifty hilar glomeruli were scored under high power (×400) and the number of proliferating cell nuclear antigen (PCNA) positive glomerular cells was counted. Treatment with STI 571 significantly reduced the number of PCNA positive cells/glomerular cross-section (gcs). The data represent the mean ± SD for groups of 7 animals. *P< 0·05 by anova with Tukey's post hoc test.