Fig. 5.

T cell and B cell epitopes were localized at different sites on the major histocompatibility complex (MHC-I). (a) Incubation of E.G7 with unconjugated 5F1·3 in the presence of 0·5% NaN₃ and subsequent incubation, after washing, with 5F1·3–fluorescein isothiocyanate (FITC) (—), comparison with untreated E.G7 incubated with 5F1·3–FITC alone (—), control (---). (b) Incubation of E.G7 with unconjugated 5F1·3 in the presence of 0·5% NaN₃ and subsequent incubation, after washing, with PBE2–FITC (─), comparison with untreated E.G7 incubated with PBE2–FITC alone (—), control (---). (c). Incubation of E.G7 with unconjugated PBE2 in the presence of 0·5% NaN₃ and subsequent incubation, after washing, with PBE2–FITC (─), comparison with untreated E.G7 incubated with PBE2–FITC alone (—), control (---). (d) Incubation of E.G7 with unconjugated PBE2 in the presence of 0·5% NaN₃ and subsequent incubation, after washing, with 5F1·3–FITC (─), comparison with untreated E.G7 incubated with 5F1·3–FITC alone (—), control (---).