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External peptide loading to major histocompatibility complex (MHC-I). RMA-S cells incubated with ovalbumin (OVA)257−264 (SIINFEKL) were analysed by indirect immunofluorescence with 5F1·3, 20G2·56, PBE2 and secondary antibody goat (Fab′)2 anti-mouse Ig–fluorescein isothiocyanate (FITC). (a) Flow cytometry. (b) Confocal microscopy (control: propidium iodide, secondary antibody: goat (Fab′)2 anti-mouse Ig–FITC).
