Table 1.

Primers and probes.

Target	Type1,2	Code3	Tm4	Length	Sequence	Exon no.	Product size
IFN-γ	St. for	IFN-g1	57·7	29	ATGAAATATACAAGTTATATCTTGGCTTT	1	494
	St. rev	IFN-g2	69·6	26	GATGCTCTTCGACCTCGAAACAGCAT	4	494
	For	F327	59·4	23	CTGACTAATTATTCGGTAACTGACTTG	3/4	107
	Rev*	R433	60·4	19	GCGACAGTTCAGCCATCAC	4	107
	Probe	P383	65·2	24	TCCAACGCAAAGCAATACATGAAC	4
IL-4	St. for	IL-4/1	63·6	21	ATGGGTCTCACCTCCCAACTG	1	614
	St. rev	IL-4/2	56·7	20	TCAGCTCGAACACTTTGAATAT	4	614
	For	F166	60·0	20	ATCTTTGCTGCCTCCAAGAA	2/3	102
	Rev	R267	60·3	19	GCAGCGAGTGTCCTTCTCA	3	102
	Probe	P216	70·0	20	TGCGACTGTGCTCCGGCAGT	3
TGF-β	St. for	ex6F	65·8	20	GGCTGGAAGTGGATCCACGA	6	243
	St. rev	ex7R	68·0	21	GCAGGAGCGCACGATCATGTT	7	243
	For	F949	60·7	20	GGCTACCATGCCAACTTCTG	6	100
	Rev	R1048	60·3	20	CCGGGTTATGCTGGTTGTAC	7	100
	Probe	P987	68·8	21	CATTTGGAGCCTGGACACGCA	6
IL-10	St. for	F109	59·2	20	CTGCCTAACATGCTTCGAGA	1	494
	St. rev	R602	59·5	20	CCAGATCCGATTTTGGAGAC	5	494
	For	F373	60·7	20	CGCTGTCATCGATTTCTTCC	3/4	88
	Rev	R460	59·6	23	TGCCTTTCTCTTGGAGCTTATTA	4/5	88
	Probe	P405	69·9	20	GAGCAAGGCCGTGGAGCAGG	4
β-actin	St. for	F745	58·1	19	CCCTGGAGAAGAGCTACGA	4	509
	St. rev	R1253	58·2	20	TAAAGCCATGCCAATCTCAT	6	509
	For	F926	63·9	19	TTGCCGACAGGATGCAGAA	5	76
	Rev	R1001	62·3	23	TCAGGAGGAGCAATGATCATTTGAT	5/6	76
	Probe*	P954	72·7	20	TGCCCTGGCACCCAGCACAA	5

¹St., standard; For, forward; Rev, reverse.

²A slight modification of the reference sequence is indicated by (*).

³Code for primers designed for this study indicates nucleotide position from the start codon (atg).

⁴T_m, primer melting temperature, calculated with Primer 3 software using default parameters. IFN: interferon; IL: interleukin; TGF: transforming growth factor.