Table 1.
Description of the antigens used in the study.
| Antigen | Abbreviation | Characteristics | Origin |
|---|---|---|---|
| Positive controls | |||
| Phytohaemagglutinin | PHA | Non-specific positive control | Sigma, France |
| Tetanus toxoid | TT | Specific positive control | Sanofi Pasteur |
| Crude pneumococcal antigens | |||
| Strain SJ04 | SJ | Capsular serotype 9, family 2 clade 3 PspA | Laboratory of Bacteriology, Hôpital de la Croix Rousse |
| Strain Rx1 | Rx1 | Laboratory non-encapsuled strain, family 1, clade 2 PspA | UAB* |
| Strain JY2141 | JY | Laboratory non-encapsuled strain, expressing only the first 115 amino-acids of Rx1 PspA | UAB |
| Native PspA | |||
| Native full length PspA from Rx1 | FL-Rx1 | Culture supernatant of Rx1 strain | UAB |
| Full length PspA from JY2141 | FL-JY | Negative control for FL-Rx1, culture supernatant of JY strain | UAB |
| Recombinant antigens | |||
| PspA-Rx1MI | PspA1 | Truncated PspA family 1, clade 2 (AA: 1–364) | Sanofi Pasteur |
| PspA-EF3296LL | PspA2 | Truncated PspA family 2, clade 3 (AA: 1–478) | Sanofi Pasteur |
| PspA-EF5668 | PspA3 | Truncated PspA family 2, clade 4 (AA: 1–369) | Sanofi Pasteur |
| PsaA | PsaA | Conserved PsaA | Sanofi Pasteur |
*
UAB = University of Alabama at Birmingham.