Table 1.
Combination of surface antigens analysed by flow cytometry and the phenotypes they identify.
| Tube no. | FITC | PE | PerCP/CyCh. | Phenotype defined |
|---|---|---|---|---|
| 1 | IgG2a | IgG1 | IgG1 | |
| 2 | CD4 | CD25 | CD62L | CD4+ CD25+ CD62L+: T regulatory cells |
| 3 | CD4 | CD25 | CD69 | CD4+ CD25+ CD69-: T regulatory cells |
| 4 | CD28 | CD8 | CD8+ CD28−: T suppressor cells | |
| 5 | CD4 | CD152 | CD4+ CD152+: activated T regulatory cells | |
| 6 | CD4 | CD3 | CD8 | CD3+ CD4-CD8−: extrathymically derived T cells, mainly found in intestinal mucosa |
| 7 | TCR γδ | CD8αβ | CD8α | TCR γδ+ CD8αα+: extrathymically derived T cells, mainly found in intestinal mucosa |
| 8 | TCR γδ | CD122 | CD25 | CD25− CD122+: T cells reported to develop in mouse liver |
| 9 | CD7 | CD2 | CD3 | CD3− CD7+: extrathymically derived T cells, mainly found in intestinal mucosa |