Fig 5.

Phagocytosis in normal and common variable immunodeficiency (CVID) dendritic cells. Purified normal and CVID monocytes, cultured for 6 days, incubated with (a) fixed ethidium bromide stained yeast in 1 : 10 ratio for 30 min, prior to washing and analysis by flow cytometry after 24 h; (b,c,d) fluoresceinated carboxylated microparticles in a 1 : 10 ratio for 1, 2 and 3 days before washing and flow cytometry. Each peak represents an increase in the number of internalized microparticles. Data representive of 12 experiments.