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. 2006 Oct;146(1):116–123. doi: 10.1111/j.1365-2249.2006.03169.x

Fig. 1.

Fig. 1

Expression of nicotinic acetylcholine receptor (nAChR) α7 on cell surface of human peripheral monocytes. (a) Two-colour staining for monocyte marker (CD14) and α7 nAChR using fluorescein isothiocyanate (FITC)-conjugated α-bungarotoxin by flow cytometry. (b) Control staining of (a). (c) Confocal laser microscopic image of cell surface CD14 on human peripheral monocytes using phycoerythrin (PE)-conjugated anti-CD14 antibody. (d) Confocal image of α7 nAChR expression on cell surface of the monocytes. (e) Merged image of cell surface CD14 (a) and α7 nAChR (b) expressions. (f) Messenger RNA expression of human peripheral blood monocytes (PBMC) from non-smokers and human monocytic cell line (U937) by reverse transcription–polymerase chain reaction.