Fig. 1.

(a, b) Epitope specificities of autoantibodies to insulin (IAA) at clinical diagnosis of type 1 diabetes (T1D). (a) Samples of patients with newly diagnosed T1D were tested for binding to [¹²⁵]I insulin in the presence of recombinant Fab (rFab) AE9D6 (black circles) or rFab CG7C7 (white circles). Binding was compared to uncompeted binding (set as 100%). Median binding is indicated. (b) Correlation of IAA-titre and competition. Binding to [¹²⁵]I-insulin in the presence of rFab AE9D6 (black circles), CG7C7 (white circles), and D1·3 (x) is plotted against the binding to [¹²⁵]I-insulin of the analysed sample in the absence of competing rFab (presented in counts per minute).