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TrpDE, provided in trans, allows thiamine-independent growth. Growth analyses were preformed at 37°C as described in Materials and Methods. Growth of DM6808 [purF2085 gnd-181(pSU19)] (▪), DM6809 [purF2085 gnd-181(pIR-EDW)] (▵), DM6810 [purF2085 gnd-181(pIR-EDMS)] (▴), DM6811 [purF2085 gnd-181(pIR-DW)] (○), and DM6812 [purF2085 gnd-181(pIR-DMS)] (•) in glucose-adenine medium is shown.
