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. 2003 Sep;23(17):5959–5971. doi: 10.1128/MCB.23.17.5959-5971.2003

FIG. 9.

FIG. 9.

Binding of TIAR depends on U6 interaction with the element. (A) Results of psoralen cross-linking assay using wild-type enhancer (Fig. 6) and HeLa nuclear extract in the absence (lane 1) or presence (lanes 2 to 7) of increasing amounts (20, 100, and 200 ng) of anti-U6 2′-O-methyl ribo-oligonucleotide (anti-U6 oligo) U6b (lanes 2 to 4) or U6c (lanes 5 to 7). (B) A UV cross-linking-IP assay was carried out with the wild-type enhancer RNA substrate (Fig. 2A) in the absence (lane 1) or presence (lanes 2 to 7) of U6b (lanes 2 to 4) or U6c (lanes 5 to 7) oligonucleotides and an antibody specific for TIA-1 and TIAR.