FIGURE 37.

Subretinal eGFP expression. Viral particles delivered to the subretinal space infect retinal pigment epithelial cells and photoreceptor cells within the subretinal space. Lentiviral subretinal transduction was observed via GFP marker gene expression, confirming that viral infection is contained within the injection area. A, Fluorescent microscopy of a section through the retina of a subject that received a subretinal injection of lentiviral containing the GFP gene. B, Overlay of a light and fluorescent image of the same subretinal section.