FIG. 6.

Effect of invF and hilA mutations on RtsA induction of sopA, sopB, invC, and icgA fusions. The strains are ΔrtsAB7; contain pBAD30, pRtsA, pRtsB, or pRtsAB; and are ΔhilA::Cm or ΔinvF::Cm. The stains contain a lacZ transcriptional fusion to the gene specified. Overnight cultures were subcultured into LB (without salt)-ampicillin-0.2% l-arabinose and grown to an OD₆₀₀ of ∼0.6 before assay of β-galactosidase. β-Galactosidase activity units are defined as (micromoles of ONP formed minute⁻¹) × 10³/(OD₆₀₀ × milliliters of cell suspension) and are reported as means ± standard deviations, where n = 2. The strains used were plasmid-containing derivatives of JS306 through JS317. WT, wild type.