TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotypea or relevant characteristics | Deletion or cloned end pointsb | Source or referencec |
|---|---|---|---|
| Strains | |||
| 14028 | Wild-type serovar Typhimurium | ATCCd | |
| JS135 | zii-8104::Tn10dTc | 78 | |
| JS247 | Φ(tetA+-rtsA+B+)3 | 4561769-4561768 | |
| JS248 | ΔrtsA5 | 4561755-4560884 | |
| JS249 | ΔrtsB6 | 4560868-4560602 | |
| JS250 | ΔrtsAB7 | 4561769-4560602 | |
| JS251 | ΔhilA112::Cm | 3019885-3021480 | |
| JS252 | ΔhilC113::Cm | 3012135-3012976 | |
| JS253 | ΔhilD114::Cm | 3017865-3018730 | |
| JS254 | ΔinvF100::Cm | 3043931-3043290 | |
| JS255 | ΔhilC-A2914::Cm | 3012135-3021480 | |
| JS256 | ΔhilC-D2915::Cm | 3012135-3018730 | |
| JS257 | ΔslrP100::Cm | 866973-869216 | |
| JS258 | Φ(tetRA-rtsA+B+)3 PhilD111::MudJ | ||
| JS259 | Φ(tetRA-rtsA+B+)3 invC101::MudJ | ||
| JS260 | Φ(tetRA-rtsA+B+)3 sopA100::MudJ | ||
| JS261 | Φ(tetRA-rtsA+B+)3 sopB101::MudJ | ||
| JS262 | Φ(tetRA-rtsA+B+)3 icgA1::MudJ | ||
| JS263 | Φ(tetRA-rtsA+B+)3 yhgF:231:MudJ | ||
| JS264 | zii-8104::Tn10dTc PhilD111::MudJ | ||
| JS265 | zii-8104::Tn10dTc invC101::MudJ | ||
| JS266 | zii-8104::Tn10dTc sopA100::MudJ | ||
| JS267 | zii-8104::Tn10dTc sopB101::MudJ | ||
| JS268 | zii-8104::Tn10dTc icgA1::MudJ | ||
| JS269 | zii-8104::Tn10dTc yhgF231::MudJ | ||
| JS270 | ΔrtsAB7 ΔhilA112::Cm | ||
| JS271 | ΔrtsAB7 invC101::MudJ | ||
| JS272 | ΔrtsAB7 ΔhiD114::Cm | ||
| JS273 | ΔrtsAB7 Φ(hilD-lac+)114 | ||
| JS274 | ΔrtsAB7 Φ(hilC-lac+)113 | ||
| JS275 | ΔrtsAB7 Φ(hilA-lac+)112 | ||
| JS276 | ΔrtsAB7 Φ(invF-lac+)100 | ||
| JS277 | ΔrtsAB7 flhC5456::MudJ | ||
| JS278 | ΔrtsAB7 fliC5050::MudJ | ||
| JS279 | Φ(hilA-lac+)112 | ||
| JS280 | ΔrtsA5 Φ(hilA-lac+)112 | ||
| JS281 | ΔrtsB6 Φ(hilA-lac+)112 | ||
| JS282 | Φ(invF-lac+)100 | ||
| JS283 | ΔrtsA5 Φ(invF-lac+)100 | ||
| JS284 | ΔrtsB6 Φ(invF-lac+)100 | ||
| JS285 | sopA100::MudJ | ||
| JS286 | ΔrtsA5 sopA100::MudJ | ||
| JS287 | ΔrtsB6 sopA100::MudJ | ||
| JS288 | ΔrtsAB7 sopA100::MudJ | ||
| JS289 | sopB101::MudJ | ||
| JS290 | ΔrtsA5 sopB101::MudJ | ||
| JS291 | ΔrtsB6 sopB101::MudJ | ||
| JS292 | ΔrtsAB7 sopB101::MudJ | ||
| JS293 | invC101::MudJ | ||
| JS294 | ΔrtsA5 invC101::MudJ | ||
| JS295 | ΔrtsB6 invC101::MudJ | ||
| JS296 | icgA1::MudJ | ||
| JS297 | ΔrtsA5 icgA1::MudJ | ||
| JS298 | ΔrtsB6 icgA1::MudJ | ||
| JS299 | ΔrtsAB7 icgA1::MudJ | ||
| JS300 | ΔrtsAB7 ΔhilC113::Cm Φ(hilA-lac+)112 | ||
| JS301 | ΔrtsAB7 ΔhiD114::Cm Φ(hilA-lac+)112 | ||
| JS302 | ΔrtsAB7 ΔhilC-D2915::Cm Φ(hilA-lac+)112 | ||
| JS303 | ΔrtsAB7 ΔhilA112::Cm Φ(invF-lac+)100 | ||
| JS304 | ΔrtsAB7 ΔhilC-D2915::Cm Φ(invF-lac+)100 | ||
| JS305 | ΔrtsAB7 ΔhilC-A2914::Cm Φ(invF-lac+)100 | ||
| JS306 | ΔrtsAB7 sopA100::MudJ | ||
| JS307 | ΔrtsAB7 ΔhilA112::Cm sopA100::MudJ | ||
| JS308 | ΔrtsAB7 ΔinvF100::Cm sopA100::MudJ | ||
| JS309 | ΔrtsAB7 sopB101::MudJ | ||
| JS310 | ΔrtsAB7 ΔhilA112::Cm sopB101::MudJ | ||
| JS311 | ΔrtsAB7 ΔinvF100::Cm sopB101::MudJ | /PICK> | |
| JS312 | ΔrtsAB7 invC101::MudJ | ||
| JS313 | ΔrtsAB7 ΔhilA112::Cm invC101::MudJ | ||
| JS314 | ΔrtsAB7 ΔinvF100::Cm invC101::MudJ | ||
| JS315 | ΔrtsAB7 icgA1::MudJ | ||
| JS316 | ΔrtsAB7 ΔhilA112::Cm icgA1::MudJ | ||
| JS317 | ΔrtsAB7 ΔinvF100::Cm icgA1::MudJ | ||
| JS318 | ΔrtsAB7 Φ(slrP-lac+)100 | ||
| JS319 | ΔrtsAB7 ΔhilC-D2915::Cm Φ(slrP-lac+)100 | ||
| JS320 | ΔrtsAB7 ΔinvF100::Cm Φ(slrP-lac+)100 | ||
| JS321 | ΔrtsAB7 ΔhilA112::Cm Φ(slrP-lac+)100 | ||
| JS322 | ΔrtsA5 ΔhilC-D2915::Cm Φ(hilA-lac+)112 | ||
| JS323 | ΔrtsA5 ΔhilC-D2915::Cm Φ(slrP-lac+)100 | ||
| JS324 | Φ(rtsA-lac+)5 | ||
| JS325 | Φ(rtsB-lac+)6 | ||
| TT10286 | LT2 hisD9953::MudJ his-9944::MudI | 40 | |
| TH1077 | LT2 fliC5050::MudJ | 34 | |
| TH4054 | LT2 flhC5456::MudJ | 18 | |
| DH5αλpir | E. coli K-12 endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lac-argF)U169 deoR φ80 Δ(lac)M15 λpir+ | Lab stock | |
| MG1655 | E. coli K-12 F− λ−ilvG rfb-50 rph-1 | Lab stock | |
| Plasmids | |||
| pKD46 | bla PBADgam bet exo pSC101 oriTS | 23 | |
| pCP20 | bla cat cI857 λPRflp pSC101 oriTS | 15 | |
| pKD3 | bla FRT cat FRT PS1 PS2 oriR6K | 23 | |
| pKD13 | bla FRT ahp FRT PS1 PS4 oriR6K (for creating in-frame deletions) | 23 | |
| pCE36 | ahp FRT lacZY+ this oriR6K | 27 | |
| pCE37 | ahp FRT lacZY+ this oriR6K | 27 | |
| pBAD30 | bla araC PBAD pACYC184 ori | 36 | |
| pCE46 | bla araC λAttL1 ccdB+cat λAttL2 pACYC184 ori | ||
| pRtsA | bla PBAD λAttB1 rtsA+ λAttB2 pACYC184 ori | 4561766-4560885 | |
| pRtsB | bla PBAD λAttB1 rtsB+ λAttB2 pACYC184 ori | 4560890-4560595 | |
| pRtsAB | bla PBAD λAttB1 rtsA+B+ λAttB2 pACYC184 ori | 4561766-4560595 | |
| pCE81 | bla PBAD λAttB1 myc-rtsA λAttB2 pACYC184 ori | 4561737-4560885 | |
| pCE82 | bla PBAD λAttB1 myc-rtsB λAttB2 pACYC184 ori | 4560850-4560595 | |
| pLS118 | bla PBADhilD-myc-His pACYC184 ori | 72 | |
| pLS119 | bla PBADhilC-myc-His pACYC184 ori | 72 |
a
Unless otherwise noted, all strains are isogenic derivatives of 14028.
b
Numbers indicate the base pairs (inclusive) that are deleted (for strains) or cloned (for plasmids), as defined in the S. enterica serovar Typhimurium LT2 genome sequence in the National Center for Biotechnology Information database.
c
This study, unless otherwise noted.
d
ATCC, American Type Culture Collection.