TABLE 1.

Kinetic properties of GlmA_Tk toward various chitooligosaccharides^a

Substrate	Vmax (U/mg)	Km (mM)	kcat/Km (M−1 s−1)
GlcN2	98.7 ± 1.7	1.37 ± 0.06	1.08 × 105
GlcN3	34.5 ± 2.6	0.270 ± 0.052	1.92 × 105
GlcN4	41.7 ± 1.0	0.295 ± 0.021	2.12 × 105
GlcN5	12.0 ± 0.2	0.0778 ± 0.0049	2.31 × 105
GlcN6	35.1 ± 0.2	0.365 ± 0.005	1.45 × 105

^aGlcNase assays were performed as described in Materials and Methods.