TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| E. coli K-12 | ||
| MC1061 | hsdR hsdM araD139 Δ(ara-leu)7679 Δlac(IPOZYA) galU galK rpsL | 34 |
| CCI18 | ΔphoA20 galE galK thi rpsE rpoB recA1 argE(Am) ΔlacX74 | 18 |
| HW1273 | CC118 harboring pHW1273 | 14 |
| Plasmids | ||
| pHW735 | pJK282 + BamHI fragment carryng most of ipaB::Tn3-lac of pHB76, Δ(invE virF)Kmr | 37 |
| pHW749 | Both invE and virF cloned into pHSG 415, Cmr | 37 |
| pHW1273 | Large virulence plasmid pSS120 of S. sonnei HW383 carrying Tn1, Apr | 14 |
| pKD46::Cm | λ Red helper plasmid:oriR101 repA101(Ts)P-araB-gam-bet-exo Cmr | This study |
| pKD13 | Kmr gene with FRT sequence | 7 |
| pCP20 | FLP helper plasmid; pSC101 replicon (Ts) bla+cat+ Flp(λRp) c1857 Apr Cmr | 7 |
| pTT0606 | pHW1273 with region upstream of icsB promote deleted from −1740 to −136 (using primers H2 and H5 for disruption; see Table 2) | This study |
| pTT0818 | pHW1273 with region upstream of icsB promoter deleted from −1740 to −41 (using primers H2 and H6 for disruption; see Table 2) | This study |
| pTT1405 | pHW1273 with region upstream of icsB promoter deleted from −1740 to −74 (using the primers H2 and H10 for disruption; see Table 2) | This study |
| pTT1608 | pHW1273 with region upstream of icsB promoter deleted from −1740 to −84 (using primers H2 and H11 for disruption; see Table 2) | This study |
| pTT1804 | pHW1273 with region upstream of icsB promoter deleted from −1740 to −94 (using primers H2 and H12 for disruption; see Table 2) | This study |
| pTT2002 | pHW1273 with region upstream of icsB promoter deleted from −1740 to −104 (using primers H2 and H13 for disruption; see Table 2) | This study |
| pTT2201 | pHW1273 with region upstream of icsB promoter deleted from −1740 to −85 (using primers H2 and H13-1 for disruption; see Table 2) | This study |
| pTT2202 | pHW1273 with region upstream of icsB promoter deleted from −1740 to −86 (using primers H2 and H13-2 for disruption; see Table 2) | This study |
| pTT2203 | pHW1273 with region upstream of icsB promoter deleted from −1740 to −87 (using primers H2 and H13-3 for disruption; see Table 2) | This study |
| pGEX-5X-3 | Expression vector, tacP lacIqSj26 Apr | 33 |
| pQW2001 | pGEX-5X-3 + a fragment encoding full-length of InvE protein, Apr | This study |